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The R group attached to the amide nitrogen contains either a carboxylate or an amine group, while pH extremes can be generated by the incorporation of a sulfonic acid group or a quaternary amine into the R group The distance between the amide bond and the ionizable or buffering group is great enough that the incorporation of the monomer into the gel matrix does not signi cantly affect the pK of the buffering group Once cast, these gels can be prepared in quantity, dried, and stored, so that a rehydration step just prior to use yields gels with reproducible gradients..

Clarification is required then, given that this confusion is sometimes fostered by certain application integration solution providers In their offerings, under the same formalism and the same data entry screen, they group both the content of an integration and the modeling of the business process However, neither the rhythms of modification, nor the user profiles, nor the technical and business preoccupations are the same 341 Business process level and integration level If we refer back to our classification of integration problem types, then propagating data and ensuring its consistency essentially require functions for transporting and adapting information (transformation/routing) For simplicity, we refer to these functions as the integration level We will use the term business process level to refer to the management of multi-step processes necessitating the orchestration of business activities with the service level provided by BPM As we saw in section 331.

Besides the obvious easy access to medical care, roads near hospitals tend to be the first ones cleared after a major snow or ice storm, making access to nearby buildings that much easier Is it a low-key site Some buildings are famous sites, like New York s World Trade Center, and while that makes them prestigious addresses, it also makes them more prone to a malicious attack If your DR site is in a building with another company s sign on the outside, you could find yourself in trouble because of their relationships or political standing It s generally best not to let the world know the location of your DR site.

Is it big enough Is the site big enough for your current operations, or is there a danger that growth at the primary site will outstrip the capacity of the DR site Do you just need space for computers, or do you need workspace, conference rooms, offices, or desks Like the New York Board of Trade, which we discuss in 21, does your business have unique space requirements that would make it difficult to relocate just anywhere.

Figure 11.6. Experimental arrangement for casting an immobilized pH gradient gel. A linear pH gradient is generated by mixing two solutions with initially equal volumes, where one solution is dense and the other is, and they are titrated to the extremes of the desired pH interval. The compensating rod in the reservoir is used as a stirrer after the addition of catalysts and for hydrostatically equilibrating the two solutions.

20

One end user that I dealt with in the Midwest United States proudly told me that their DR site is a safe 10 miles away from their main site. Since the disaster they are most concerned about is tornadoes, that sounded pretty reasonable to this lifelong resident of the East Coast. Then they sheepishly admitted that both sites are right in the heart of the same tornado alley, and despite the distance, they felt one tornado could conceivably wipe out both sites. Evan

TABLE 11.1. Monomers Used for the Preparation of IPG gelsa pK 1.2 3.1 3.6 4.4 4.6 6.2 Name 2-Acrylamido-2-methylpropanesulfonic acid 2-Acrylamidoglycolic acid N-Acryloylglycine 3-Acrylamidopropanoic acid 4-Acrylamidobutyric acid 2-Morpholinoethylacrylamide Rb C(CH3)2(CH2SO3H) CH(OH)COOH 2COOH CH (CH2)2COOH (CH2)3COOH MW 207 145 129 143 157 184