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Figure 14.8. Bioelution of LDH isoenzymes from a Sepharose AMP af nity column using a concave NADH gradient. [Reprinted, by permission, from P. Brodelius and K. Mosbach, FEBS Lett. 35, 1973, 223 226. Separation of the Isoenzymes of Lactate Dehydrogenase by Af nity Chromatography Using an Immobilized AMP-Analogue . The exclusive # for all languages and countries is vested in the Federation of European Biochemical Societies.] 21 rdlc pdf 417 PDF417 Barcode Creating Library for RDLC Reports | Generate ...
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Free trial package available to insert PDF - 417 barcode image into Client Report RDLC . generate an N 6 -(6-aminohexyl)adenosine-50 -monophosphate linkage. This type of separation may be accomplished using biospeci c elution methods, but would not be possible with nonspeci c elution, where signi cant changes in the structures of all of the isoenzymes may be expected to occur during elution, yielding one major elution zone. 14.7.3. Determination of Association Constants by High-Performance Af nity Chromatography10 Association constants for the reactions of biomolecules with their binding partners can be evaluated using af nity chromatography. Basically, an immobilized af nity ligand competes with a soluble ligand for the binding sites of the biomolecule, and the retention volume of the biomolecule is measured as a function of the concentration of soluble ligand. Samples injected contain a xed concentration of the biomolecule, and varying concentrations of soluble ligand. The theoretical approach to relate retention volumes to association constants is based on a model that consists of a monomeric biomolecule that has a single bind0 ing site (e.g., it is applicable to the Fab fragments of antibodies, but not to intact immunoglobulins). The biomolecule interacts with an immobilized ligand and a soluble ligand, each of which also has a single binding site. The model is depicted in Figure 14.9. Because of the general applicability of the model, the biomolecule is represented as A, and the immobilized ligand and soluble ligand are shown by the symbols X and L, respectively. The species to consider in developing the relationships are therefore A, X, L, AX, and AL, and they are related through rdlc pdf 417 How to add Barcode to Local Reports ( RDLC ) before report ...
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