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orientation or spacing of the ligands may also lead to reduced af nity, so that immobilization conditions must be optimized. Figure 14.7 illustrates the effects of some of these factors.8 Many support materials are commercially available in a preactivated form, requiring only the coupling of the desired af nity ligand. These materials already have a spacer arm incorporated, and possess an active functionality for ligand immobilization. Table 14.4 lists a number of reactions of activated supports with ligand functional groups. For all of these illustrated reactions, the R group of the ligand may be a small molecule, such as a hapten or inhibitor, or a macromolecule, such as a protein, carbohydrate, or nucleic acid that has been modi ed, if necessary, to possess the indicated functional group. 14.7.2. Elution Methods Elution methods are classi ed as either biospeci c or nonspeci c. Biospeci c elution is accomplished by the addition of a low molecular weight inhibitor or competitor to the mobile phase, causing competitive binding. For example, the binding between immobilized glucosamine and a lectin may be reversed by the addition of glucose as a modi er to the mobile phase; glucose in the eluent interacts with the lectin binding sites, and a lectin glucose complex is eluted. The immobilized glucosamine and the soluble glucose in the eluent compete for lectin (analyte) binding sites. On the other hand, if the lectin is immobilized for the puri cation of a glycoprotein, glucose may again be used as a mobile-phase modi er, but in this case the glucose will displace the glycoprotein from the column by competition with the

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21

TABLE 14.4. Reactions of Activated Support Materials with Af nity Ligands Activation Method Cyanogen bromide Reaction with Af nity Ligand

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The time since the recovery has been a very interesting one for Pat and Steve on many levels As a direct result of their efforts, they have been rewarded by NYBOT, with Pat having been made Senior Executive Vice President of NYBOT, and later named Interim Chief Operating Officer of the entire organization Steve was made NYBOT s Chief Information Officer Even before things settled down at NYBOT, Pat and Steve started to become major industry celebrities In the month of September 2001 alone, just the first three weeks after the terrorist attacks, NYBOT was written up over 230 times in publications and on newswires around the world, ranging from the Wall Street Journal and Fortune Magazine to The People s Daily in China, the Times of London, the National Post in Canada, and even Progressive Farmer magazine.

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it imposed the Internet as the preferred (and often, the only) network for these exchanges; it helped bring about the emergence of XML, a new standard for the description of information (see section 3211); it led to the appearance of a large number of new actors disputing the definition of norms for exchanged documents and the provision of value-added sector-based or even inter sector networks In the domain of inter-enterprise exchanges, numerous initiatives have driven XML to become a new standard for document exchange Among these initiatives we would cite principally: UBL (Universal Business Language): the result of work carried out internationally in a technical committee of OASIS (Organization for the Advancement of Structured Information Standards) defining the library of standard electronic commercial documents (purchase orders, invoices, etc) and using XML.

In the months that followed, NYBOT s recovery was written up in thousands of periodicals, and was shown on television programs all around the world Then the conference invitations started to come in Pat and Steve have spoken at conferences put on by The Federal Reserve Bank, The Futures Industry Association (four times), The Commodities Futures Trading Commission (three times), The Vanguard Technology Transfer Institute, and at two different VERITAS Software conferences In their year-end awards for 2001, Information Week magazine named Steve one of their co-CIOs of the Year, citing all the work that he and Pat put into their business recovery plans They also made a point of contrasting the frantic pace of work at NYBOT with Steve s easygoing manner In the months that followed, requests for interviews and appearances have continued.

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During the week of September 11, 2002, Pat appeared on CNNfn, CNBC, the BBC, and New York City s NY1 cable news channel, and NYBOT was written up once again in dozens of newspapers and periodicals around the world NYBOT, led by Pat, has begun to lobby the CFTC to get all exchanges and member organizations up to certain levels of disaster preparedness They believe that these organizations have an obligation to their customers and to the general public (who are, after all, the ultimate consumers of these commodities) to ensure that they survive disasters Pat has taken slight advantage of some of his speaking engagements and has publicly lobbied to make these changes.

Though the CFTC did not take maximum advantage of the attention that they and their efforts received during Y2K preparation to promote disaster preparedness, the CFTC has used Pat well at CFTC-sponsored forums to promote disaster preparedness, and it is hoped they will be able to get appropriate regulations in place sooner rather than later Shortly after the recovery was in full swing and it was clear that NYBOT would, in fact, survive, Pat Gambaro received what he believes to be the nicest compliment of all, one that put all of their efforts into perspective Don t just think about how your efforts managed to keep the exchange trading, Pat was.

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