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The rst step after column preparation is the application of the sample; during this step, the enzyme that binds to the inhibitor is retained, or adsorbed on the column This is followed by a rinse step, whereby all nonbinding species are removed The third step involves elution, and this step.

16. On platform 7, the flow manager delivery service (adapter) deposits the file in the reception space of application E, Client Accounting. On platform 8, it deposits the file in the reception space of application F, General Accounting. The flow manager thus constitutes the backbone of enterprise IT. It is thus nothing less than the implementation of the exchange process sub-level described in section 3.4.3. At the same time, beyond the exchanges between the applications in its information system, the enterprise will also have to get control over the numerous exchanges that it must carry out with its partners, clients, suppliers or administrations. This is the domain of inter-enterprise exchanges, Business to Business (B to B, or B2B). 4.3. Integrating inter-enterprise exchanges Inter-enterprise exchanges are today the domain where the problem of application integration is most crucial. This is a question of bringing the applications of the enterprise IT into communication with the applications of an external information system as part of commercial transactions. Let us consider the case of a client/supplier relationship between two enterprises A and B. The exchanges to be established in this case can, for example, take the form illustrated in Figure 4.7.

The first step in NYBOT s future DR plans was taken very quickly: They have separated their data center from the building in which the trading floor is located. The attacks simultaneously cost them both their trading floor and their data center. By putting the two in separate buildings, NYBOT hopes that a single event can no longer disable both functions. The current plan is for NYBOT to hold on to the Long Island City site for the time being, and perhaps permanently, depending on how their other plans go. But they have other options. One DR option is to merge their DR plan with the Merc s. The Merc has their DR building on Long Island,1 and NYBOT could take some space and move their DR environment there. Another idea is to speak with other exchanges in other parts of the United States, and discuss the possibility of trading DR space with them. In this model, NYBOT might wind up with a DR trading floor in Chicago, while a Chicago exchange uses some of NYBOT s space in either Long Island City or 1 North End. Steve acknowledges that traveling to Chicago could be very difficult in some circumstances, so a remote DR site would serve as an additional DR site rather than the primary one. At this writing, these decisions have not yet been made.

Long Island City is not located on Long Island, but rather is part of Queens, one of the five boroughs of New York City. Long Island is located several miles east of Queens.

TABLE 14.3. General Af nity Ligands and Their Binding Partnersa Ligand(s) Cibachron blue dye, or derivatives of AMP, the NADH, or NADPH Lectins: concanavalin A, lentil lectin, wheat germ lectin con gurations Trypsin inhibitor, methyl esters of amino acids, or D-amino acids Phenylboronic acid Selectivity Dehydrogenase enzymes, through binding at NAD(P)H binding site Polysaccharides, glycoproteins, glycolipids, and membrane proteins with certain sugar Proteases Species containing cis-diol groups, such as glycosylated hemoglobins, sugars, and nucleic acids Immunoglobulins, binding through Fc fragment Nucleases, polymerases, nucleic acids

Perhaps the most exciting thing about the move for Pat and Steve was that since they got to redesign the trading space from scratch, they got to investigate and implement new technologies to accelerate and enhance the trading process.