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As expensive and unpleasant as the direct costs of downtime are, the indirect costs can be significantly higher and have much greater long-term impact on the enterprise Imagine that you wish to order an $80 sweater from one of those catalog clothing stores via telephone (or the Internet) When you call, the operator politely tells you that their systems are down, so could you please call back in a couple of hours But you want your sweater now4 So you call a different catalog company and order your sweater there instead The direct loss to Company 1 is clear; it is the price of the sweater that you didn t buy from them But there are other, more indirect costs You took your business to its competitor; this helps the competitor, so not only did Company 1 lose $80, but Company 2 made an extra $80.

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Assuming that Company 2 serviced your order satisfactorily, which company will you call next time you want a sweater Most likely, Company 2 More losses to Company 1 And when your friends ask you where you got that nice new sweater, and you tell them Company 2, the losses to Company 1 increase They increase further if you tell the whole story to your friends, convincing them to take their business to Company 2 Multiply that by the number of customers who may call during the system outage, and you can see that the impact is significant, though perhaps impossible to quantify precisely The following are some examples of indirect costs: Customer satisfaction As in the preceding example, if a customer visits a web site intending to purchase something, or to take part in the activities that the web site delivers, and he cannot, he will surely be frustrated.

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6. The C-PAGE electropherogram shown (Fig. 12.14) below was obtained following the separation of products of the dideoxy (Sanger) DNA sequencing reactions for a polydeoxynucleotide. A 12-base primer labeled with uorescein isothiocyanate was used to initiate the four DNA polymerase reactions in the 30 ! 50 direction. The products of the four reactions that included ddGTP, ddATP, ddCTP, and ddTTP were, respectively, combined in the volume ratio 8:4:2:1. Using the electropherogram, determine the sequence of the rst 13 bases that follow the primer. Remembering that the sequence of the original polydeoxynucleotide is complementary to the sequence of the dideoxy products detected, give the 50 ! 30 sequence of this section of the original polydeoxynucleotide. 7. Capillary isoelectric focusing was performed in a 75-mm i. d. capillary where the surface silanols had been derivatized with linear polyacrylamide. A mixture of proteins having pI values between 6 and 8 were focused using ampholytes covering the pH 3 10 range, using an anolyte of 20 mM HCl and a catholyte of 20 mM NaOH. A static separation pattern was indicated by a current minimum that occurred after 90 min at 15 kV. How can salt mobilization be used to elute the separation pattern at the anodic end of the capillary

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Depending on his past experience, he may decide to go away and do other things, returning to the site later If he is a first-time visitor to the site, he may never return If he has experienced downtime on other occasions, he may or may not ever return If he is a happy customer, he will likely return later But there are few better ways to turn happy customers into unhappy ones than repeating bad experiences Remember the rule of thumb: It costs 10 times as much to bring in a new customer as it does to retain an old one Bad publicity and press Although the news media s feeding frenzy has diminished from 1999 and 2000, when large companies web sites experience significant downtime, it still makes the news, though generally not the front page.

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