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SEC mobile phase composition is, in principle, not an important factor In practice, however, electrostatic effects may be important Under physiological conditions (09% NaCl), Donnan effects are usually negligible The eluent pH may be altered to change the net charge on macromolecules, to minimize Donnan effects For example, the separation of nucleic acids is normally performed at low pH, so that some phosphate groups are protonated In some cases, charged groups on the stationary phase may be chemically removed prior to column packing For protein separations, denaturing additives, such as urea or guanidinium HCl, are used to produce random coils of polypeptides for molecular weight determinations SDS and disul de bond reducing agents may also be added to the mobile phase Finally, the viscosity of the samples may be important if macromolecule concentrations are high enough to contribute to band broadening as a result of unstable ow 146.

In early 2002, the trader transition area was relocated from the first floor to the second floor, and a whole new trading floor was built out in its place. Normally, the building of a new trading floor can be expected to take 6 months or more, but they were able to accelerate the process, and completed the floor in just 2 months. *****

21

GEL MATRICES FOR SIZE EXCLUSION CHROMATOGRAPHY6 Gels used for bioanalytical SEC must have hydrophilic properties Polysaccharides, such as dextran and agarose, as well as synthetic polymers, such as polyacrylamide, have been used successfully for SEC The monomers and low molecular weight polymer forms of these polymers are water soluble, and must be cross-linked to create insoluble gel beads Some types of gels may be dried and rewetted reversibly, such as dextran These are called aerogels, and are known to maintain their open structure when they are dehydrated Xerogels, on the other hand, shrink when dried, and swell when they are rewetted, so that alterations in pore size may occur Table 142 lists some of the commercially available SEC stationary-phase materials, along with their molecular weight fractionation ranges for globular proteins The trade names of the stationary phase media represent particular polymers formed under well-controlled conditions.

On platform 5, the flow manager delivery service (adapter) deposits the file from application A in the reception space for the accounting application D 6 Application C in the means-of-payment system creates a file that contains the events in debit dates for amortizable loans 7 The acquisition service (adapter) of the flow manager takes charge of this file on platform 3 8 The flow manager transport service sends the file generated by application C to platform 5, which hosts the aggregation service 9 The transport service on platform 5 receives the file from application C 10 On platform 5, the flow manager aggregation service aggregates elements from the three files, and creates a file from the results 11 The flow manager s transport service sends the file that results from this aggregation to platform 6, which hosts the transformation service 12.

In its new location, the transition area spread out to cover a lot more space, and included many of the amenities that the traders had done without since the relocation. The additional space gave the traders and support staff telephones, PCs, and space and equipment where they could enter their trades, as well as a lounge, a coat room, a vending machine area, offices for the executives, and space for other critical functions like compliance and trade clearing. The addition of the new trading rings brought the total number of rings to eight. These new rings made trading much easier and more comfortable and meant that commodities could trade more hours a day than at any time since 9/11. For example, cocoa trades for nearly four hours a day, or only 1 hour less than at the WTC. Eight rings, of course, is not 13, and so the commodities were not all able to trade in the hours of the day that they would like. The cotton and orange juice traders would prefer to trade with morning hours, but are unable to, due to the lack of trading space. Doubling up on trading space with other commodities is still a procedural requirement.

SephadexTM consists of dextran, crosslinked with epichlorohydrin in alkaline solution SephacrylTM is an allyl dextran, cross-linked with N,N 0 -methylenebis(acrylamide) SepharoseTM is a beaded form of agarose gel, where aggregates of the polysaccharide chains are formed..