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Since information technology costs were still a concern, when Steve and Pat approached the Board to ask for additional funding to expand the Queens site to accommodate the new commodities and personnel acquired in the merger, they were unable to get the money This meant that the recovery site did not grow to support the additional capacity that was necessary to keep all of NYBOT s products running after a disaster The last major shortcoming in the plan was in the recoverability of their back office applications They knew that they could switch over all of their trading operations to the Queens site in about 15 minutes However, without the ability to recover back office operations, they could not close their trading day, or perform other important, but less critical functions.

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capillary for the detection of catecholamines, and detection limits of 200 500 amol were obtained (for S/N 2) When the capillary diameter was reduced to 127 mm, the same 5-mm carbon ber working electrode yielded detection limits of 5 25 amol, almost two orders of magnitude lower The improvement results from the collection ef ciency of the working electrode The smaller id capillary allows less analyte to exit the capillary without being electrochemically converted7 This result contrasts directly with those obtained with optical detectors, where detection limits are worse for smaller capillaries because less detectable analyte is present in the light path Using a microinjector for electrokinetic microsampling, this instrument has been used for the detection of catecholamines present in the intracellular uid of single nerve cells8 Figure 12.

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Because of the ongoing budget tightness, Steve and Pat had to develop a low-cost or cost-free scheme to back up these functions One of the compromises that they made at the recovery site to save money was that there would be limited space for NYBOT s staff to work The plan was that some of them would report to work at the recovery site, while others would work from home The administrators were given suitable access from home (paid for in full by NYBOT), and would be expected to report there in the event of a disaster This would be inconvenient, but since the system administrative staff s presence was not considered critical to the business, this made sense The Long Island City facility held limited space for offices, trading pits, facilities, and all of the other things that NYBOT would need to operate long-term.

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10 shows a diagram of the injection apparatus and an electropherogram generated with the working electrode poised at 750 mV versus SSCE, to oxidize catecholamines To date, amperometric detectors have only been applied to small, readily oxidized analyte species A variety of alternate amperometric detection schemes are under development, however, including indirect amperometry With this technique, the separation buffer contains a redox active species that is continuously detected by the ultramicroelectrode The analyte displaces the electroactive species, so that the electrochemical response is decreased during the elution of an analyte peak 1254 Radiochemical Detection9 Radiochemical CE detectors have been developed to test the application of CE to conventional DNA sequencing techniques, where the detection of 32P provides the sequence information (see Section 108) The 32P decomposes according to Eq 1211, emitting a b-particle (electron) that can be detected using scintillators..

There was another implicit assumption being made throughout NYBOT s disaster plans, which was most evident in this lack of space for system administrators Long Island City was seen as a temporary home The plan did not consider the possibility that the entire World Trade Center building might be lost They figured that if there were a fire or other calamity, they might be out of their WTC building for a week or two, or a month at most They did not consider the possibility that the World Trade Center could be totally lost Needless to say, this assumption would not prove to be a valid one..

This ad hoc approach, which integrates a single application or software package, presents major inconveniences, such as not pooling the cost of the integrations required for each individual project, and often, multiplying the number of tools required by these integrations. This explains why projects that implement a single new application often drag on the coattails of projects that genuinely implement a global exchange infrastructure, where the implemented solutions and thus their costs are pooled. This subsequently results in the launch of a more ambitious project to restructure the entire information system. This is a classic bottom-up approach in pragmatic programs.

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