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10.8. DNA SEQUENCING WITH THE MAXAM GILBERT METHOD11 Determining the sequence of an unknown DNA sample may be accomplished using either enzymatic (the dideoxy method) or chemical (the Maxam Gilbert method) reactions that yield a series of detectable fragments that differ in length by a single base. The Maxam Gilbert method uses four chemical reactions that selectively cleaves DNA at dG, dC, dG dA and dC dT residues, and the products of these partial cleavage reactions are subjected to polyacrylamide gel electrophoresis. The initial step in the sequencing protocol involves labelling single-stranded DNA with 32P at either the 50 - or the 30 -end (but not both). The labeled DNA is then divided into four portions, and subjected to four separate cleavage reactions. Each reaction results in partial cleavage of the fragment at particular bases, and, following PAGE, the sequence of the DNA is read from the pattern of bands on the autoradiogram. For example, given an initial fragment, labeled at the 50 -end, that has the sequence 32P-GCTGCTAGGTGCCGAGC, partial cleavage at (and removal of) the G residues will yield the following detectable fragments:

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One step above database administrator directed log transfers on the replication food chain are commercial database replication management products. Some, such as Oracle Replication Server, are offered as part of the database management system, and others, such as Quest SharePlex, are third-party products. To replicate databases, a database replication manager application walks through the log files and copies the transactions to one or more replica databases. Instead of relying on the recovery manager to roll the logs, the replication manager mimics an application performing the workload represented

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by the log files. The process that picks apart the log files and reconstructs the transaction is commonly called a transfer manager, and it can write to one or more target machines that have a local replay manager acting as a database application. Replication is typically done asynchronously; that is, the log files are scanned by the manager after the transaction has already been applied to the primary system, and without any locking or checkpointing to ensure that the transfer manager and the primary application coordinate updates to all databases. As a result, it s possible for the transfer manager to be several transactions behind the primary application database, slowed by the time required to walk the logs and apply transactions to the secondary machines. As with log replay, there are design considerations for database replication managers:

3.3.1.3. Groups of elements Groups of elements can be used to assemble the actions performed by each participant of a process. A Pool (Figure 3.34) represents a participant in a process, which can be an organizational entity in an enterprise (customer services, accounting, etc.) or more generally a role (purchaser, vendor, etc.).

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The relationship between insert rate, update rate, and the log transfer manager s ability to extract transactions must be clearly understood. Under high insert and update conditions, does the transfer manager run into contention for access to the log files The longest delay you ll see under extreme conditions must be known. One way to accomplish this is to watch the number of transactions that go into the log transfer manager queue. If you can only replicate 10 transactions a second but have insert peaks of 50 transactions a second, you ll push 40 transactions a second off into the transfer manager. You may end up with a delay of a few minutes while waiting for the replicated databases to absorb these load peaks. Some database replication managers can fail completely once the load exceeds a certain level. These solutions are not appropriate for largescale operations with heavy-duty levels of transactions. Speak to your vendor and determine their rated limits for transaction throughput. What happens when the primary database fails Does the transfer manager fail as well, requiring applications to do the ask then restart recovery outlined previously If the transfer manager continues to apply updates, how can a client application determine when it s acceptable to connect to another copy of the database When the primary returns to service, what happens to the log transfer manager The secondary database (now acting as the primary) has probably reinserted all of the transactions that were entered into the queue in the transfer manager at the time of the primary failure. Should the transfer manager flush its queue Should it remain quiescent until restarted by the database administrator

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