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3.5.2.2. Distributed architecture A distributed architecture shares out the infrastructure over the set of machines that support the applications to be integrated, with each application connecting locally to that infrastructure through adapters. This architecture does not introduce any single point of failure or contention. However, on the downside, it requires tools adapted to the heterogenity of the platforms where its components must be deployed, as well as tools for supervising and maintaining those components on the various platforms.

A host that is trunked to a switch has a single IP address, much like the redundant NIC case. The switch and host handle the scatter and gather of packets distributed over the multiple network links, distributing data round-robin on each end. Because all of the links are active, trunking increases the bandwidth between switch and host. Furthermore, trunking software handles failures of the links transparently, so any NIC or cable breakdown is hidden. A caution with trunking is that frequently it requires devices that have several network connections on the same card. This card then becomes a single point of failure. Of course, you can combine methods and trunk over two cards to two different switches, using the multiple network attach approach described in the previous section. Given that SPOF caveat, though, trunking is an excellent choice for alleviating server input bottlenecks while gaining protection from the most common network failures cables and connectors that are subject to physical wear and tear.

a Reprinted, with permission, from J. B. Fenn, M. Mann, C. K. Meng, S. F. Wong, and C. M. Whitehouse, Science 246, 1989, 64 71. Electrospray Ionization for Mass Spectrometry of Large Biomolecules . Copyright # 1989 by AAAS.

Up to this point, we ve looked at single failures between switches and hubs and servers Aside from cable damage, network failures tend to fall into the excessive load, configuration error, or denial-of-service categories, where protection requires separate, redundant networks When the primary network is deemed unusable for any reason, all traffic switches to the secondary network If you re suffering from inconsistencies in configuration or severe load problems, turning on the clean network may be the best and most expedient way to restore order among the network citizens A word of warning: Load-related problems simply move to the redundant network and cause headaches there; configuration problems can be resolved by going to a clean area where they don t exist and then taking the time to diagnose the sick network Redundant networks are commonly called rails (borrowing from the AC power distribution vernacular).

When wiring a data center, independent power rails bring AC to the machine room from different circuits, and possibly from different sources as well Similar design considerations are used in data center equipment with hot-swap and redundant power supplies Rail switching within power supplies allows a server to switch to an alternate source of line current without interruption The same notion of rail switching applies to taking all IP traffic off of one network and sending it over the redundant set of network rails The most difficult part of multiple network configuration is sorting out the IP addressing on each network The simplest approach to multiple networks is to use multiple switches, with each switch connected to every server.

1252 Mass Spectrometric Detection Mass spectrometric detectors for capillary electrophoresis are necessarily postcolumn detectors and must be interfaced to the cathodic end of the capillary These detectors consist of four main components: the interface, that joins the capillary to the ion source, the ion source, that generates ionic fragments from neutral analyte species, the mass analyzer, that distinguishes ions by their mass/charge (m/z) values, and the ion detector, that measures and ampli es the signal The principles and instrumentation of bioanalytical MS are explained in 15 Soft ionization methods produce few fragments under relatively mild conditions The ionization method that has received the most attention in terms of its applicability to protein and DNA analysis is the electrospray ionization (ES) technique This is a soft method that is capable of generating molecular ions from biological macromolecules present in solution Table 12.

In an asymmetric configuration, one set of switches acts as hot standby for the active set, and in the event of a failure, all interfaces on all servers failover to the second set of switches This is essentially the redundant network attach on steroids, and it may prove more efficient to insist that all hosts failover.