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portion of the separation pattern is visualized, and individual DNA differences are readily observed. Figure 10.8 illustrates DNA ngerprinting for paternity testing. The separation pattern resulting from the child s DNA can be seen to contain bands present in both the mother s and the father s DNA, while a new band in the child s DNA suggests a possible new mutation. The critical reagent in RFLP methods is the restriction enzyme (or cocktail of enzymes), which must produce very distinct fragment lengths for individual DNA samples. Diagnostic RFLP methods employ DNA that is readily available from the white cells of patient blood samples. Forensic methods, on the other hand, use DNA samples of unpredictable (usually low) quantity; furthermore, if the DNA sample is old and has been exposed to sunlight (UV radiation), the DNA may be present as cleaved fragments rather than intact genes. In such cases, the available DNA is ampli ed in concentration using the polymerase chain reaction (PCR).10 PCR technology has revolutionized DNA analysis, and has made such general forensic tests feasible.

Database replication buys you protection against several disasters: corruption of your primary database, failure of an operational system when the MTTR is measured in seconds not minutes, and in some cases protection from a fullfledged physical disaster. Database replication can rely on variations of file distribution tricks, using log replay, built-in database features such as distributed transaction management, or third-party transaction processing and queuing systems that guarantee delivery of transactions to multiple database instances on multiple servers.

- events. Inclusive decision/merge (OR): multiple sequences can be executed. Parallel fork/join: several sequences are executed in parallel, or indication of a joining point between multiple sequences. Table 3.2. BPMN: graphical representation of actions

Database log replay is the simplest approach to database replication. Taking a page from the filesystem replication playbook, a database logs all changes to permanent structures, typically in an undo or redo log that is used for

PCR ampli cation of DNA uses repetitive thermal cycling between three temperatures to (a) dissociate analyte DNA into single strands, (b) anneal primers, and (c) biosynthesize new DNA with the enzyme DNA polymerase A solution containing the double-stranded DNA of interest, an excess of two unique primer oligonucleotides, a heat-stable DNA polymerase (Taq DNA polymerase is used; it is isolated from the thermophilic bacterium Thermus aquaticus), and an excess of deoxynucleotide triphosphates, is thermally cycled as follows At 93 94 C, the analyte DNA is denatured into single strands The temperature is rapidly shifted to an annealing temperature between 37 and 72 C (the best temperature is determined experimentally for a particular set of primers), and at this temperature, the single strands anneal, or form heteroduplexes, with the primers The primers are single-stranded, 20 26 bases in length and 40 60% GC content.

rolling the transaction forward in the event of a system failure Log replay involves manually (or via a scheduled script) copying these log files to another machine, and then reapplying the transactions from the logs to the replica databases using the recovery manager, creating a similar copy of the primary database with some minor changes, including, but not limited to, timestamps and transaction IDs One concern with log-replay-style replication is that it is possible for some transactions to fall into the gutter between the primary and secondary database machines, if the primary crashes and takes its log files with it before the final transactions are copied from the log If you replay the log file, then accept five new transactions in the primary database, then suffer a crash, those five transactions won t make it to the secondary server.

Two unique primers are needed for PCR ampli cation, with one annealing to each strand, near the 30 -end of the region of interest Following the annealing step, the temperature is rapidly changed to 70 72 C, where the DNA polymerase sequentially extends the primers one nucleotide at a time, moving toward the 50 -end of the region of interest The nucleotides that are incorporated into the new double strands are complementary in sequence to the analyte strands After this cycle, one copy of each analyte DNA molecule has been produced Repeated thermal cycling through the denaturation, annealing and extension temperatures yields copies of the copies, and an exponential ampli cation of the DNA concentration In principle, the number of copies produced is equal to 2n 1, where n is the number of complete thermal cycles used Most PCR protocols specify over 25 cycles.

Another concern with log replay is that the database on the remote side is not, in fact, identical to the one on the primary side As a result, it would be more difficult, and, in fact, probably impossible, to take an extract from it and apply that extract back to the primary side, in the event of data corruption As we mentioned previously, timestamps and record identifiers are going to be different on the remote side If it were necessary to copy the replicated database back to the primary database server, serious confusion and corruption will result However, database log replay is a relatively big win because it requires no application changes to implement replication The database administrators can configure the log copy and recovery steps, setting the time interval between copies and the verification of updates on the replicated machines.