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Figure 11.8. Comparison of (a) carrier ampholyte (pH 4 6 range) and (b) IPG (pH 4 8 range) isoelectric focusing pattern stability over time, for the separation of a mixture of bean seed proteins.11 [Reprinted, with permission, from Methodology of two-dimensional electrophoresis with immobilized pH gradients for the analysis of cell lysates and tissue proteins , A. Gorg, W. Postel, A. Domscheit, and S. Gunther, in Two-Dimensional Electrophoresis , Proceedings of the International Two-Dimensional Electrophoresis Conference, Vienna, November 1988. A. T. Endler and S. Hanash, Eds., VCH Publishers, Weinheim, Germany, 1989. # 1988 by VCH Verlagsgesellschaft mbH.]

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One of the outputs that management may expect from a DR plan is a specific timeline. OK, they ll say, the disaster has hit. When will my application be back up Unfortunately, it doesn t work quite that way. The reality is that everything always takes longer than you think it will, especially in a crisis situation when you don t know how many people, which people, or what skills you ll have at your disposal during the recovery period. Timelines that obligate the recovery team to complete certain tasks in a particular time window are much too specific to circumstances. Unless a lot of extra time is built into the timelines, they are surefire recipes for failure, but worse than that, they are recipes for incredibly visible failure. After all, in a disaster recovery, everybody s watching. Their jobs (and yours, for that matter) may depend on your ability to get the systems working again. A much better approach, and one that is more likely to succeed, is to develop critical paths to success. Rather than saying that task A will take an hour, task B will take 35 minutes, and task C will take 8 hours and 19 minutes, develop a set of critical paths for tasks, as shown in Figure 20.2. Microsoft Project is an excellent example of a tool that can generate critical paths and help determine which tasks need to be performed in what order, while also keeping track of costs and deadlines. By arranging critical paths for tasks, you can determine where personnel shortages may occur before they actually do, enabling you to bring more people into the teams that may create bottlenecks.

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As we have been saying throughout this chapter, it is important to assign teams to tasks, rather than assigning individuals. An individual may not be available during a disaster or may behave in unpredictable ways in the face of all the unexpected stress that is coming his way. Therefore, it is best to assign small teams to tasks. Each team should have a leader and a basic hierarchy. That hierarchy may just include the designation of a backup for the leader, although some organizations may choose to assign complete hierarchies from top to bottom.

systems, since gel-to-gel reproducibility is high when gels are initially prepared in large quantities. The high-resolution and separation stability afforded by isoelectric focusing on immobilized pH gradient gels has also been exploited in 2D electrophoresis methods, where IEF in the rst dimension is followed by a second separation method performed in a perpendicular direction.

Assign people to do the things they are best at. Sometimes you ll have senior people in your organization who have moved on from a task that they were very good at so that they can learn new skills. This is an excellent thing to do

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11.5. TWO-DIMENSIONAL ELECTROPHORESIS12 Two-dimensional separation techniques are those in which a sample is subjected to two displacement processes that occur orthogonally to each other. The two dimensions should be based upon two completely different separation mechanisms, since identical mechanisms merely yield diagonal separation patterns. The resolving power of 2D techniques can be expressed by Eq. 11.3: n2 n2 1 11:3

. . . . .

to advance one s career, but in a disaster it is quite reasonable to ask these people to return to their previous roles, at least for the duration of the crisis, if they are the best people for the job In addition to a team leader, who coordinates all specific tasks, there must be a team spokesperson The spokesperson is the only team member who is authorized to speak to the overall recovery coordinator or anyone else outside the team If all team members answer questions and communicate outside of the group, they are not performing the tasks that will bring the organization back online What s more, doing so will add a great deal of confusion, as mixed messages reach the outside world.

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